Review



genorm housekeeping gene selection kit for rattus norvegicus  (PrimerDesign Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    PrimerDesign Inc genorm housekeeping gene selection kit for rattus norvegicus
    Genorm Housekeeping Gene Selection Kit For Rattus Norvegicus, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genorm+housekeeping+gene+selection+kit+for+rattus+norvegicus/genorm+housekeeping+gene+selection+kit+for+rattus+norvegicus/pmc03998187-109-15-17
    Average 90 stars, based on 1 article reviews
    genorm housekeeping gene selection kit for rattus norvegicus - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Gene Expression:

    Article Title: Inhibition of bladder tumour growth by sirolimus in an experimental carcinogenesis model
    Article Snippet: Reactions were carried out in a thermocycler Gene Amp PCR System 9600 (Perkin Elmer, Norwalk, CO, USA), for 10 min at 25 ° C, 50 min at 50 ° C and 5 min at 85 ° C. Reaction products were then digested with 1 μ L RNase H for 20 min at 37 ° C and, finally, cDNA eluted to a final volume of 50 μ L and stored at − 20 ° C. Relative gene expression was quantified by real-time PCR using the LightCycler® 480 II (Roche, Rotkreuz, Switzerland). .. A normalization step preceded the quantification of gene expression, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal reference genes for the present study [13]; the selected genes were glyceraldehyde phosphate dehydrogenase (Gapdh) and ribosomal protein L13 (Rpl13). .. Real-time PCR reactions used QuantiTect® Primer Assay (Qiagen, Hilden, Germany) for the gene of interest, caspase 3, and the endogenous controls selected previously (Table 1), together with QuantiTect SYBR Green PCR Kit Gene expression.

    Article Title: Sitagliptin prevents aggravation of endocrine and exocrine pancreatic damage in the Zucker Diabetic Fatty rat - focus on amelioration of metabolic profile and tissue cytoprotective properties
    Article Snippet: Performed using 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes to this study [ ]. .. Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for Bax, Bcl2, TRB3, IL-1β, PCNA and VEGF.

    Article Title: Cardiac antiapoptotic and proproliferative effect of recombinant human erythropoietin in a moderate stage of chronic renal failure in the rat
    Article Snippet: Performed using 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes to this study. .. [ ] Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for TGF-β1 and PCNA, as proliferative markers; VEGF as an angiogenesis marker; a synthase 2 (inducible) and 3 (constitutive, endothelial) from nitric oxide, NOS2 and NOS3 as indicators of endothelial and constitutive enzyme activity; Cytochrome c as a vascular damage factor; IL-2, NF-κB, and TNF-α as inflammatory markers; and at least apoptotic indicators, such as caspase 9, caspase 3, Bax, Bcl2, Fas and Fas ligand.

    Article Title: A role for transforming growth factor-beta apoptotic signaling pathway in liver injury induced by ingestion of water contaminated with high levels of Cr(VI).
    Article Snippet: Hexavalent chromium [Cr(VI)] exposure is commonly associated with lung cancer.. Although other adverse health effects have been reported, some authors, on assuming that orally ingested Cr(VI) is efficiently detoxified upon reduction by body fluids, believe that Cr(VI) do not target cells other than respiratory tract cells.. In rodents, ingested Cr(VI)-contaminated water was reported to induce, in the liver, increases in TGF-β transcripts.

    Article Title: Recombinant human erythropoietin treatment protects the cardio-renal axis in a model of moderate chronic renal failure
    Article Snippet: Chronic kidney disease (CKD) patients develop anemia because of the low kidney erythropoietin (EPO) production, thus promoting cardiovascular complications.. The degree of renal insufficiency might determine the moment to start recombinant human erythropoietin (rhEPO) therapy, but the molecular basis for these options deserves better elucidation.. This study aimed to clarify the cardio-renal effects of earlier rhEPO therapy in rats with moderate chronic renal failure (CRF).

    Article Title: Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat: Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers
    Article Snippet: Gene expression was performed using a 7900 HT Sequence Detection System (Applied Biosystems, Foster City, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes for this study [ ]. .. Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for transforming growth factor beta 1 (QT00187796), proliferating cell nuclear antigen (QT00178647), mechanistic target of rapamycin (QT00180586), nuclear factor kappa B (QT01577975), monoclonal antibody Ki-67 (QT00450786), and tumor protein p53 (QT00193522) as proliferative markers; vascular endothelial growth factor beta (QT01290163) as angiogenic marker; interleukin 1 beta (QT00181657), interleukin 2 (QT00185360), tumor necrosis factor (QT00178717), cyclooxygenase 2 (QT00192934), and C-reactive protein (QT00391650) as inflammatory markers.

    Selection:

    Article Title: Inhibition of bladder tumour growth by sirolimus in an experimental carcinogenesis model
    Article Snippet: Reactions were carried out in a thermocycler Gene Amp PCR System 9600 (Perkin Elmer, Norwalk, CO, USA), for 10 min at 25 ° C, 50 min at 50 ° C and 5 min at 85 ° C. Reaction products were then digested with 1 μ L RNase H for 20 min at 37 ° C and, finally, cDNA eluted to a final volume of 50 μ L and stored at − 20 ° C. Relative gene expression was quantified by real-time PCR using the LightCycler® 480 II (Roche, Rotkreuz, Switzerland). .. A normalization step preceded the quantification of gene expression, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal reference genes for the present study [13]; the selected genes were glyceraldehyde phosphate dehydrogenase (Gapdh) and ribosomal protein L13 (Rpl13). .. Real-time PCR reactions used QuantiTect® Primer Assay (Qiagen, Hilden, Germany) for the gene of interest, caspase 3, and the endogenous controls selected previously (Table 1), together with QuantiTect SYBR Green PCR Kit Gene expression.

    Article Title: Sitagliptin prevents aggravation of endocrine and exocrine pancreatic damage in the Zucker Diabetic Fatty rat - focus on amelioration of metabolic profile and tissue cytoprotective properties
    Article Snippet: Performed using 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes to this study [ ]. .. Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for Bax, Bcl2, TRB3, IL-1β, PCNA and VEGF.

    Article Title: Cardiac antiapoptotic and proproliferative effect of recombinant human erythropoietin in a moderate stage of chronic renal failure in the rat
    Article Snippet: Performed using 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes to this study. .. [ ] Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for TGF-β1 and PCNA, as proliferative markers; VEGF as an angiogenesis marker; a synthase 2 (inducible) and 3 (constitutive, endothelial) from nitric oxide, NOS2 and NOS3 as indicators of endothelial and constitutive enzyme activity; Cytochrome c as a vascular damage factor; IL-2, NF-κB, and TNF-α as inflammatory markers; and at least apoptotic indicators, such as caspase 9, caspase 3, Bax, Bcl2, Fas and Fas ligand.

    Article Title: A role for transforming growth factor-beta apoptotic signaling pathway in liver injury induced by ingestion of water contaminated with high levels of Cr(VI).
    Article Snippet: Hexavalent chromium [Cr(VI)] exposure is commonly associated with lung cancer.. Although other adverse health effects have been reported, some authors, on assuming that orally ingested Cr(VI) is efficiently detoxified upon reduction by body fluids, believe that Cr(VI) do not target cells other than respiratory tract cells.. In rodents, ingested Cr(VI)-contaminated water was reported to induce, in the liver, increases in TGF-β transcripts.

    Article Title: Recombinant human erythropoietin treatment protects the cardio-renal axis in a model of moderate chronic renal failure
    Article Snippet: Chronic kidney disease (CKD) patients develop anemia because of the low kidney erythropoietin (EPO) production, thus promoting cardiovascular complications.. The degree of renal insufficiency might determine the moment to start recombinant human erythropoietin (rhEPO) therapy, but the molecular basis for these options deserves better elucidation.. This study aimed to clarify the cardio-renal effects of earlier rhEPO therapy in rats with moderate chronic renal failure (CRF).

    Article Title: Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat: Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers
    Article Snippet: Gene expression was performed using a 7900 HT Sequence Detection System (Applied Biosystems, Foster City, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes for this study [ ]. .. Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for transforming growth factor beta 1 (QT00187796), proliferating cell nuclear antigen (QT00178647), mechanistic target of rapamycin (QT00180586), nuclear factor kappa B (QT01577975), monoclonal antibody Ki-67 (QT00450786), and tumor protein p53 (QT00193522) as proliferative markers; vascular endothelial growth factor beta (QT01290163) as angiogenic marker; interleukin 1 beta (QT00181657), interleukin 2 (QT00185360), tumor necrosis factor (QT00178717), cyclooxygenase 2 (QT00192934), and C-reactive protein (QT00391650) as inflammatory markers.

    Software:

    Article Title: Inhibition of bladder tumour growth by sirolimus in an experimental carcinogenesis model
    Article Snippet: Reactions were carried out in a thermocycler Gene Amp PCR System 9600 (Perkin Elmer, Norwalk, CO, USA), for 10 min at 25 ° C, 50 min at 50 ° C and 5 min at 85 ° C. Reaction products were then digested with 1 μ L RNase H for 20 min at 37 ° C and, finally, cDNA eluted to a final volume of 50 μ L and stored at − 20 ° C. Relative gene expression was quantified by real-time PCR using the LightCycler® 480 II (Roche, Rotkreuz, Switzerland). .. A normalization step preceded the quantification of gene expression, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal reference genes for the present study [13]; the selected genes were glyceraldehyde phosphate dehydrogenase (Gapdh) and ribosomal protein L13 (Rpl13). .. Real-time PCR reactions used QuantiTect® Primer Assay (Qiagen, Hilden, Germany) for the gene of interest, caspase 3, and the endogenous controls selected previously (Table 1), together with QuantiTect SYBR Green PCR Kit Gene expression.

    Article Title: Sitagliptin prevents aggravation of endocrine and exocrine pancreatic damage in the Zucker Diabetic Fatty rat - focus on amelioration of metabolic profile and tissue cytoprotective properties
    Article Snippet: Performed using 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes to this study [ ]. .. Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for Bax, Bcl2, TRB3, IL-1β, PCNA and VEGF.

    Article Title: Cardiac antiapoptotic and proproliferative effect of recombinant human erythropoietin in a moderate stage of chronic renal failure in the rat
    Article Snippet: Performed using 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes to this study. .. [ ] Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for TGF-β1 and PCNA, as proliferative markers; VEGF as an angiogenesis marker; a synthase 2 (inducible) and 3 (constitutive, endothelial) from nitric oxide, NOS2 and NOS3 as indicators of endothelial and constitutive enzyme activity; Cytochrome c as a vascular damage factor; IL-2, NF-κB, and TNF-α as inflammatory markers; and at least apoptotic indicators, such as caspase 9, caspase 3, Bax, Bcl2, Fas and Fas ligand.

    Article Title: A role for transforming growth factor-beta apoptotic signaling pathway in liver injury induced by ingestion of water contaminated with high levels of Cr(VI).
    Article Snippet: Hexavalent chromium [Cr(VI)] exposure is commonly associated with lung cancer.. Although other adverse health effects have been reported, some authors, on assuming that orally ingested Cr(VI) is efficiently detoxified upon reduction by body fluids, believe that Cr(VI) do not target cells other than respiratory tract cells.. In rodents, ingested Cr(VI)-contaminated water was reported to induce, in the liver, increases in TGF-β transcripts.

    Article Title: Recombinant human erythropoietin treatment protects the cardio-renal axis in a model of moderate chronic renal failure
    Article Snippet: Chronic kidney disease (CKD) patients develop anemia because of the low kidney erythropoietin (EPO) production, thus promoting cardiovascular complications.. The degree of renal insufficiency might determine the moment to start recombinant human erythropoietin (rhEPO) therapy, but the molecular basis for these options deserves better elucidation.. This study aimed to clarify the cardio-renal effects of earlier rhEPO therapy in rats with moderate chronic renal failure (CRF).

    Article Title: Conversion to Sirolimus Ameliorates Cyclosporine-Induced Nephropathy in the Rat: Focus on Serum, Urine, Gene, and Protein Renal Expression Biomarkers
    Article Snippet: Gene expression was performed using a 7900 HT Sequence Detection System (Applied Biosystems, Foster City, USA). .. A normalization step preceded the gene expression quantification, using geNorm Housekeeping Gene Selection kit for Rattus norvegicus (Primer Design, Southampton, UK) and geNorm software (Ghent University Hospital, Center for Medical Genetics, Ghent, Belgium) to select optimal housekeeping genes for this study [ ]. .. Real-time PCR reactions used specific QuantiTect Primer Assays (Qiagen, Hilden, Germany) with optimized primers for transforming growth factor beta 1 (QT00187796), proliferating cell nuclear antigen (QT00178647), mechanistic target of rapamycin (QT00180586), nuclear factor kappa B (QT01577975), monoclonal antibody Ki-67 (QT00450786), and tumor protein p53 (QT00193522) as proliferative markers; vascular endothelial growth factor beta (QT01290163) as angiogenic marker; interleukin 1 beta (QT00181657), interleukin 2 (QT00185360), tumor necrosis factor (QT00178717), cyclooxygenase 2 (QT00192934), and C-reactive protein (QT00391650) as inflammatory markers.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Recombinant human erythropoietin treatment protects the cardio-renal axis in a model of moderate chronic renal failure
    Article Snippet: Chronic kidney disease (CKD) patients develop anemia because of the low kidney erythropoietin (EPO) production, thus promoting cardiovascular complications.. The degree of renal insufficiency might determine the moment to start recombinant human erythropoietin (rhEPO) therapy, but the molecular basis for these options deserves better elucidation.. This study aimed to clarify the cardio-renal effects of earlier rhEPO therapy in rats with moderate chronic renal failure (CRF).

    SYBR Green Assay:

    Article Title: Recombinant human erythropoietin treatment protects the cardio-renal axis in a model of moderate chronic renal failure
    Article Snippet: Chronic kidney disease (CKD) patients develop anemia because of the low kidney erythropoietin (EPO) production, thus promoting cardiovascular complications.. The degree of renal insufficiency might determine the moment to start recombinant human erythropoietin (rhEPO) therapy, but the molecular basis for these options deserves better elucidation.. This study aimed to clarify the cardio-renal effects of earlier rhEPO therapy in rats with moderate chronic renal failure (CRF).

    Polymerase Chain Reaction:

    Article Title: Recombinant human erythropoietin treatment protects the cardio-renal axis in a model of moderate chronic renal failure
    Article Snippet: Chronic kidney disease (CKD) patients develop anemia because of the low kidney erythropoietin (EPO) production, thus promoting cardiovascular complications.. The degree of renal insufficiency might determine the moment to start recombinant human erythropoietin (rhEPO) therapy, but the molecular basis for these options deserves better elucidation.. This study aimed to clarify the cardio-renal effects of earlier rhEPO therapy in rats with moderate chronic renal failure (CRF).



    Similar Products

    90
    PrimerDesign Inc genorm housekeeping gene selection kit for rattus norvegicus
    Genorm Housekeeping Gene Selection Kit For Rattus Norvegicus, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genorm+housekeeping+gene+selection+kit+for+rattus+norvegicus/genorm+housekeeping+gene+selection+kit+for+rattus+norvegicus/pmc03998187-109-15-17
    Average 90 stars, based on 1 article reviews
    genorm housekeeping gene selection kit for rattus norvegicus - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results